Isolation and Identification of Bacteriophage Specific Salmonella typhi from Chicken Skin

Sri Hardanti, Agustin Krisna Wardani, Widya Dwi Rukmi
  JTP, pp. 107-116  

Abstract


Salmonella enterica serovar Thypi is a gram-negative bacteria that causes typhoid fever very endemic in Indonesia. The use of uncontrolled and irrational antibiotics leads to resistance cases that result to ineffective treatment. This problems are  requiring more effective, efficient, environmentally friendly and safe handling alternatives. The aim of this research is to get bacteriophage specific Salmonella typhi from chicken skin sample and characterize phage produced morphologically. Isolation of bacteriophage using plaque assay method with double layer technique in this research has succeeded in isolating phage specific Salmonella typhi named KAS with final concentration of 1.1 x 109 PFU / mL. Based on the observation of the plaque formed, the KAS phage is categorized as litic phage. The results of the confirmation test of phage KAS in liquid medium showed that phage KAS has varied ability in decreasing number of pathogenic bacteria Salmonella typhi with MOI value 101, 102 and 104. Transmission Electron Microscopy (TEM) revealed phage KAS has morphological characteristics similar to Podoviridae

Keywords


typhoid fever, biocontrol, lysis, plaque assay

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