KARAKTERISTIK KIMIA EKSTRAK ETANOL DAUN TENGGULUN (Protium javanicum) BERDASARKAN TINGKAT KETUAAN DAUN
DOI:
https://doi.org/10.21776/ub.jtp.2024.025.01.6Keywords:
Ekstrak, Fenol, Flavonoid, Tanin, TenggulunAbstract
Ekstrak etanol daun tenggulun mengandung sejumlah senyawa bioaktif yang berpotensi sebagai antioksidan. Tingkat ketuaan daun adalah salah satu komponen yang mempengaruhi jumlah dan komposisi senyawa fitokimia dalam ekstrak daun tenggulun. Tujuan dari penelitian ini adalah untuk menentukan tingkat ketuaan daun yang ideal sehingga ekstrak yang dihasilkan memiliki karakteristik kimia terbaik. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan tingkat ketuaan daun tenggulun yaitu P1 (daun pucuk), P2 (daun muda), P3 (daun dewasa), dan P4 (daun tua). Perlakuan diulang sebanyak 4 kali sehingga diperoleh 16 unit percobaan. Hasil penelitian menunjukkan bahwa tingkat ketuaan daun berpengaruh nyata (P<0,05) terhadap rendemen, kadar total fenol, total flavonoid, total tanin dan nilai IC50 ekstrak etanol daun tenggulun. Perlakuan terbaik diperoleh dari ekstrak etanol daun tenggulun dewasa dengan nilai rendemen 18,812%, total fenol 22,612 mg GAE/g, total flavonoid 4,330 mg QE/g, total tanin 23,392 mg TAE/g dan IC50 21,265 ppm. Terdapat hubungan korelasi negatif yang cukup antara total fenol dengan aktivitas antioksidan yang ditunjukkan dengan nilai koefisien korelasi (R) sebesar -0,70.
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