Amobilisasi Sel Pediococcus Acidilactici F11 Penghasil Bakteriosin Pada Gel Kalsium Alginat
Immobilized cell Pediococcus acidilactici F11 that can produce bacteriocin in calciumalginate gel was studied. Â This research was conducted to observe the ability of immobilized cell toproduce bacteriocin in TGE broth with initial pH 6,5 in a shaker waterbath at 100 rpm and 37Cfor 24 hours.Â The cell was immobilized by dropping the mixed of 3% sodium alginate with microbial cells to0,1 M calcium chloride solution. Â More than 99% of cell introduced was successfully immobilized.Â The cell density in beads was in the range of 1,4 x 109Â to 1,6 x 1010Â cfu /g beads.Â The ability of immobilized cell and free cell to produce bacteriocin was similar qualitatively. Â Theyalso showed similar pH profile during the time of fermentation.
Keywords: Â immobilized cell, Pediococcus acidilactici F11, bacteriocin, calcium alginate gel
LicenseAuthors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal