EFFECT OF PURPLE SWEET POTATO (Ipomoea batatas) EXTRACT AND FERMENTATION TIME IN WATER KEFIR CHEMICAL PROPERTIES
Keywords:
Anthocyanin, Chemical Properties, Fermentation, Purple Sweet Potato, Water KefirAbstract
As a bacteria starter, water kefir is commonly made from water, sugar, dried or fresh fruits, and kefir grains. Purple sweet potato (PSP) contains natural anthocyanins and carbohydrates as colouring agents and carbon sources in water kefir drinks production. This study determines the effect of the PSP extract addition, fermentation time, and their interaction in the water kefir. The study was based on chemical properties such as total anthocyanins, antioxidant activity IC50, total acid, total sugar, degree of acidity (pH), and total dissolved solids (TDS). The method used Factorial Randomized Group Design with 0%, 10%, 30%, and 50% (v/v) of addition of PSP extract and 0, 24, and 48 hours of fermentation time as factors. The results were analyzed using Analysis of Variance (ANOVA) with a 5% confidence interval. The results showed that PSP extract addition and fermentation time significantly affected all chemical properties analysis and their interaction. The best treatment was retrieved with 50% PSP extract addition and 48 hours of fermentation time with total anthocyanin 193.197 ± 3.255 ppm, antioxidant activity IC50 460.620 ± 20.973 ppm, total acid 10.429 ± 0.371%, total sugar 1.356 ± 0.058%, pH 2.3 ± 0.058, and TDS 2.1 ± 0.2 ºBrix
References
Affandi, D, -R., Handajani, -S. 2011. Es krim ubi jalar ungu (Ipomoea batatas) : tinjauan sifat sensoris, fisik, kimia, dan aktivitas antioksidannya. Jurnal Teknologi Hasil Pertanian. 4, 94–103. https://doi.org/10.20961/jthp.v0i0.13572
Afifah, N. 2010. Analisis Kondisi dan Potensi Lama Fermentasi Medium Kombucha (Teh, Kopi, Rosela) Dalam Menghambat Pertumbuhan Bakteri Patogen (Vibrio cholerae dan Bacillus cereus). Skripsi. Universitas Islam Negeri Maulana Malik Ibrahim. Malang
Alsayadi, -M., AlJawfi, -Y., Belarbi, -M., Sabri, F, -Z., 2013. Antioxidant Potency ff Water Kefir. Journal of Microbiology, Biotechnology and Food Sciences. 2, 2444–2447. https://www.jmbfs.org/wp-content/uploads/2013/05/jmbfs-0310-alsayadi.pdf
Dewi, L, -R., Laksmiani, N, P, -L., Paramita, N, L, P, -V., Wirasuta, I, M, A, -G. 2014. Uji aktivitas antioksidan ekstrak etanol kulit ubi jalar ungu (Ipomoea batatas (L.) Lam) dengan metode ferrous ion chelating (FIC). Jurnal Farmasi Udayana. 3, 14–17.
https://ojs.unud.ac.id/index.php/jfu/article/view/10795
Francavilla, -A., Joye, I, -J. 2020. Anthocyanins in whole grain cereals and their potential effect on health. Nutrients. 12, 2922. https://doi.org/10.3390/nu12102922
Gulitz, -A., Stadie, -J., Wenning, -M., Ehrmann, M, -A., Vogel, R, -F. 2011. The microbial diversity of water kefir. International Journal of Food Microbiology. 151, 284–288. https://doi.org/10.1016/j.ijfoodmicro.2011.09.016
Hambali, -M., Mayasari, -F., Oermansyah, -F., 2014. Ekstraksi antosianin dari ubi jalar dengan variasi konsentrasi solven, dan lama waktu ekstraksi. Jurnal Teknik Kimia. 20, 25–35. http://jtk.unsri.ac.id/index.php/jtk/article/view/171
Hardoko, -H., Hendarto, -L., Siregar, T, -M. 2010. Pemanfaatan ubi jalar ungu (Ipomoea batatas L. Poir) sebagai pengganti sebagian tepung terigu dan sumber antioksidan pada roti tawar. Jurnal Teknologi dan Industri Pangan. 2, 25–32. https://journal.ipb.ac.id/index.php/jtip/article/view/2414
Huang, D, -J., Lin, C, -D., Chen, H, -J., Lin, Y, -H. 2004. Antioxidant and antiproliferative activities of sweet potato (Ipomoea batatas [L.] Lam ’Tainong 57’) constituents. Botanical Bulletin of Academia Sinica. 45, 179–186. https://www.researchgate.net/publication/244478484_Antioxidant_and_antiproliferative_activities_of_sweet_potato_Ipomoea_batatas_L_Lam_%27Tainong_57%27_constituents
Husna, N, -El, Novita, -M., Rohaya, -S. 2013. Kandungan antosianin dan aktivitas antioksidan ubi jalar ungu segar dan produk olahannya. Agritech. 33, 296–302. https://doi.org/10.22146/agritech.9551
Ismawan, E. 2003. Pemanfaatan Limbah Rumput Laut Kapphaphycus alvarezii dalam Proses Pembuatan Sirup Jeruk (Citrus aurantium). Skripsi. IPB University. Bogor
Kano, -M., Takayanagi, -T., Harada, -K., Makino, -K., Ishikawa, -F. 2005. Antioxidative activity of anthocyanins from purple sweet potato, Ipomoera batatas Cultivar Ayamurasaki. Bioscience, Biotechnology, and Biochemistry. 69, 979–988. https://doi.org/10.1271/bbb.69.979
Kunaepah, U. 2008. Pengaruh Lama Fermentasi dan Konsentrasi Glukosa Terhadap Aktivitas Antibakteri, Polifenol Total dan Mutu Kimia Kefir Susu Kacang Merah. Thesis. Universitas Diponegoro. Semarang
Lestari, M, -W., Bintoro, V, -P., Rizqiati, -H. 2018. Pengaruh lama fermentasi terhadap tingkat keasaman, viskositas, kadar alkohol, dan mutu hedonik kefir air kelapa. Jurnal Teknologi Pangan 2, 8–13. https://doi.org/10.14710/jtp.v2i1.20750
Maharani, B, -C., Lindriati, -T., Diniyah, -N. 2016. Pengaruh variasi waktu blanching dan konsentrasi asam sitrat terhadap karakteristik dan aktivitas ekstrak pigmen ubi jalar ungu (Ipomoea batatas L.). Jurnal Penelitian Pangan. 1, 60–67. https://doi.org/10.24198/jp2.2016.vol1.1.10
Montilla, E, -C., Hillebrand, -S., Winterhalter, -P. 2011. Anthocyanins in purple sweet potato (Ipomoea batatas L.) varieties. Fruit, Vegetable and Cereal Science and Biotechnology. 5, 19–24. https://www.researchgate.net/publication/283429468_Anthocyanins_in_purple_sweet_potato_Ipomoea_batatas_L_Varieties
Mubin, M, -F., Zubaidah, -E. 2016. Studi pembuatan kefir nira siwalan (Borassus flabellifer L.) (pengaruh pengenceran nira siwalan dan metode inkubasi). Jurnal Pangan dan Agroindustri. 4, 291–301. https://jpa.ub.ac.id/index.php/jpa/article/view/330
Ningsih, -R., Rizqianti, -H., Nurwantoro, -N. 2019. Total padatan terlarut, viskositas, total asam, kadar alkohol, dan mutu hedonik water kefir semangka dengan lama fermentasi yang berbeda. Jurnal Teknologi Pangan. 3, 325–331. https://doi.org/10.14710/jtp.v3i2.24151
Nintami, A, -L., Rustanti, -N. 2012. Kadar serat, aktivitas antioksidan, amilosa, dan uji kesukaan mi basah dengan substitusi tepung ubi jalar ungu (Ipomoea batatas var Ayamurasaki) bagi penderita diabetes melitus Tipe-2. Journal of Nutrition College. 1, 382–387. https://doi.org/10.14710/jnc.v1i1.679
Pratiwi, B, -M., Rizqiati, -H., Pratama, -Y. 2018. Pengaruh substitusi buah naga merah terhadap aktivitas antioksidan, pH, total bakteri asam laktat dan organoleptik kefir sari kedelai. Jurnal Teknologi Pangan. 2, 98–104. https://doi.org/10.14710/jtp.v2i2.20638
Purwanti, I. 2013. Uji Total Asam dan Organoleptik dalam Pembuatan Yoghurt Susu Kacang Hijau (Phaseolus radiatus) dengan Penambahan Ekstrak Ubi Jalar Ungu (Ipomoea batatas L). Skripsi. Universitas Muhammadiyah Surakarta. Surakarta.
Qi, -Q., Chu, -M., Yu, -X., Xie, -Y., Li, -Y., Du, -Y., Liu, -X., Zhang, -Z., Shi, -J., Yan, -N. 2022. Anthocyanins and proanthocyanidins: Chemical structures, food sources, bioactivities, and product development. Food Reviews International. 38, 1–29. https://doi.org/10.1080/87559129.2022.2029479
Rahmawati, I, -S., Zubaida, -E., Saparianti, -E. 2015. Evaluasi pertumbuhan isolat probiotik (L. casei dan L. plantarum) dalam medium fermentasi berbasis ubi jalar (Ipomoea Batatas L.) selama proses fermentasi (kajian jenis isolat dan jenis tepung ubi jalar). Jurnal Aplikasi Teknologi Pangan. 4, 133–141. https://doi.org/10.17728/jatp.v4i4.3
Raini, -M., Ismawati, -A. 2011. Kajian: khasiat dan keamanan stevia sebagai pemanis pengganti gula. Media Litbang Kesehatan. 21, 145–156. https://media.neliti.com/media/publications-test/150020-kajian-khasiat-dan-keamanan-stevia-sebag-a1634682.pdf
Rizky, A, -M., Zubaidah, -E. 2015. Pengaruh penambahan tepung ubi ungu jepang (Ipomea batatas L var. Ayamurasaki) terhadap sifat fisik, kimia, dan organoleptik kefir ubi ungu. Jurnal Pangan dan Agroindustri. 3, 1393–1404. https://jpa.ub.ac.id/index.php/jpa/article/view/262
Saludung, -J., Hamid, -S., Pramezwary, -A. 2020. Development evaluation of various products from purple sweet potatoes (Ipomoea Batatas L. Poir). Proceeding of International Conference on Science and Advanced Technology. 6, 1174–1187. https://ojs.unm.ac.id/icsat/article/view/17920
Sampurno, -A., Cahyanti, -A. 2015. Variasi jenis gula tebu terhadap derajat brix, pH, total asam dan kesukaan panelis pada water kefir. Jurnal Teknologi Pangan dan Hasil Pertanian. 11, 34–39.
Saragih, C., Herawati, N., Efendi, R., 2017. Pembuatan sirup ubi jalar ungu (Ipomea batatas L.) dengan penambahan sari lemon (Citrus limon L.). Jurnal Online Mahasiswa (JOM) Universitas Riau 4, 1–15. https://jom.unri.ac.id/index.php/JOMFAPERTA/article/view/16865
Suda, -I., Oki, -T., Masuda, -M., Kobayashi, -M., Nishiba, -Y., Furuta, -S. 2003. Physiological functionality of purple-fleshed sweet potatoes containing anthocyanins and their utilization in foods. JARQ: Japan Agricultural Research Quarterly. 37, 167–173. https://doi.org/10.6090/jarq.37.167
Supriono, T. 2008. Kandungan Beta Karoten, Polifenol Total dan Aktivitas “Merantas” Radikal Bebas Kefir Susu Kacang Hijau (Viginaradiata) oleh Pengaruh Jumlah Starter (Lactobacillus bulgaricus dan Candida kefir) dan Konsentrasi Glukosa. Thesis. Universitas Diponegoro. Semarang
Susanti, -I., Wijaya, -H., Hasanah, -F., Heryani, -S. 2018. Copigmentation of anthocyanin extract of purple sweet potatoes (Ipomea Batatas L.) using ferulic acid and tannic acid. IOP Conference Series: Earth and Environmental Science. 116, 012006. https://doi.org/10.1088/1755-1315/116/1/012006
Teow, C, -C., Truong, V, -D., McFeeters, R, -F., Thompson, R, -L., Pecota, K, -V., Yencho, G, -C. 2007. Antioxidant activities, phenolic and β-carotene contents of sweet potato genotypes with varying flesh colours. Food Chemistry. 103, 829–838. https://doi.org/10.1016/j.foodchem.2006.09.033
Umam, M, -F., Utami, -R., Widowati, -E. 2012. Kajian karakteristik minuman sinbiotik pisang kepok (Musa paradisiaca formatypical) dengan menggunakan starter Lactobacillus acidophilus IFO13951 Bifidobacterium longum ATCC15707. Jurnal Teknosains Pangan. 1, 1–10. https://jurnal.uns.ac.id/teknosains-pangan/article/view/4176
Wahyono, -H., Fitriani, -L., Widyaningsih, T, -D. 2015. Potensi cincau hitam (Mesona palustris Bl.) sebagai pangan fungsional untuk kesehatan: Kajian pustaka. Jurnal Pangan dan Agroindustri. 3, 957–961. https://jpa.ub.ac.id/index.php/jpa/article/view/218
Wibisanti, GA. 2018. Studi Pembuatan Kefir Nira Tebu (Saccharum officinarum L.) (Pengaruh Konsentrasi Gula Dan Lama Fermentasi). Skripsi. Universitas Brawijaya. Malang
Wicaksono, LA. 2013. Ekstraksi Antosianin dari Limbah Kulit Ubi Jalar Ungu (Ipomoea Batatas L.) Metode Microwave Assisted Extraction (Kajian Waktu Ekstraksi dan Rasio Bahan:Pelarut). Skripsi. Universitas Brawijaya. Malang
Wu, -L., Hsu, -H., Chen, -Y., Chiu, -C., Lin, -Y., Ho, J, -A. 2006. Antioxidant and antiproliferative activities of red pitaya. Food Chemistry. 95, 319–327. https://doi.org/10.1016/j.foodchem.2005.01.002
Yang, -J., Gadi, R, -L. 2008. Effects of steaming and dehydration on anthocyanins, antioxidant activity,total phenols and color characteristics of purple-fleshed sweet potatoes (Ipomoea batatas). American Journal of Food Technology. 3, 224–234. https://doi.org/10.3923/ajft.2008.224.234
Yang, -Z., Zhai, -W. 2010. Optimization of microwave-assisted extraction of anthocyanins from purple corn (Zea mays L.) cob and identification with HPLC–MS. Innovative Food Science & Emerging Technologies. 11, 470–476. https://doi.org/10.1016/j.ifset.2010.03.003
Yoshinaga, -M., Tanaka, -M., Nakatani, -M. 2000. Changes in anthocyanin content and composition of developing storage root of purple-fleshed sweet potato (Ipomoea batatas (L.) Lam). Breeding Science. 50, 59–64. https://doi.org/10.1270/jsbbs.50.59
Downloads
Published
Issue
Section
License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal