Purification of Phage Cocktail and its Inhibition Spectrum to Foodborne Disease Bacteria

Lia Oriana Nindita, Agustin Krisna Wardani


The aim of this research is to purify a phage cocktail to obtain a single phage and evaluate the broad host range of a single phage. The obtained single phage (ФLA-1), was found to attack strains of Shigella flexneri, Shigella dysentri, Eschericia coli, Salmonella thypi, and Listeria monocytogenes, but not members of the strains Staphylococcus aureus, Bacillus cereus and Lactobacillus plantarum. The data showed that ФLA-1 is more  effective to attack the Shigella flexneri than other strain with the burst size of 1.37.108 PFU/ml. It  can reduced the cell numbers of their positive bacteria targets after 8 hr. The complete nucleotide sequence of the genome 21226 basepairs of фLA-1 was determined.

Keywords: purification, phage cocktail, plaque, pathogen bacteria, foofdborne disease

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