EKSTRAKSI SENYAWA BIOAKTIF CABAI RAWIT (CAPSICUM FRUTESCENS L.) MENGGUNAKAN METODE EKSTRAKSI GELOMBANG ULTRASONIK

Authors

  • Joni Kusnadi Universitas Brawijaya
  • Dian Wuri Andayani Universitas Brawijaya
  • Elok Zubaidah Universitas Brawijaya
  • Estri Laras Arumingtyas Universitas Brawijaya

DOI:

https://doi.org/10.21776/ub.jtp.2019.020.02.1

Keywords:

Bioaktif, Cabai Rawit, Ekstraksi, Ultrasonik

Abstract

ABSTRAK

 

 Cabai rawit (Capsicum frutenses L.) memiliki kandungan senyawa bioaktif seperti capsaicinoid, fenol, flavonoid dan vitamin C yang tinggi, sehingga berpotensi sebagai sumber antioksidan. Tujuan penelitian ini untuk mengetahui pengaruh rasio bahan:pelarut dan lama ekstraksi dengan gelombang ultrasonik/Ultrasonic Assisted Extraction (UAE) terhadap kandungan fitokimia dan aktivitas antioksidan dari ekstrak cabai rawit. Faktor rasio bahan:pelarut (1:5; 1:10; dan 1:15) dan lama waktu ekstraksi (5, 10, dan 15 menit) diamati agar diperoleh karakteristik ekstrak cabai rawit terbaik. Hasil penelitian menunjukkan bahwa rasio bahan:pelarut dan lama waktu ekstraksi memberikan pengaruh nyata terhadap kandungan capsaicinoid, total fenol dan flavonoid. Perlakuan terbaik metode ekstraksi UAE diperoleh dari perbandingan bahan:pelarut = 1:10 dan lama waktu ekstraksi 15 menit dengan kandungan capsaisin 102,89 mg/g bk, total fenol 56,75 mg GAE/g bk, total flavonoid 208,89 mg QE/g bk dan kandungan vitamin C 27,57 mg/g bk.

 

ABSTRACT

 

Chilli pepper (Capsicum frutenses L.) has high content of bioactive compounds such as capsaicinoid, phenol, flavonoids and vitamin C, so that potentially as a source of antioxidants. The aim of this study to determine the effect of materials:solvent and extraction time of ultrasonic assisted extraction (UAE) to phytochemical content and antioxidant activity of the chilli pepper extract. The ratio of material:solvent (1:5; 1:10; and 1:15) and time of extraction (5, 10, dan 15 min) were observed to obtain the best characteristics of chilli pepper extract. The results showed that the ratio of material:solvent and time of extraction gave a significant effect on capsaicinoid content, total phenol and flavonoids. The best treatment was obtained from the material:solvent ratio of 1:10 and extraction time of 15 minutes with capsaisin content of 102.89 mg/g dw, total phenol of 56.75 mg GAE/g dw, total flavonoid of 208.89 mg QE/g dw and vitamin C of 27.57 mg/g dw.

References

Alupului, -A., 2012. Microwave extraction of active principles from medicinal plants. U.P.B. Science Bulletin, Series B 74(2), 29-14. https://www.scientificbulletin.upb.ro/rev_docs_arhiva/fullf4b_141413.pdf

Atanassova, -M., Georgieva, -S., Ivancheva, -K., 2011. Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. Journal of the University of Chemical Technology and Metallurgy. 46(1), 81-88. https://dl.uctm.edu/journal/node/j2011-1/12_Maria_Atanasova.pdf

Azmir, -J., Zaidul, -I., Rahman, -M., Sharif, -K., Mohamed, -A., Sahena, -F., Jahurul, -M., Ghafoor, -K., Norulaini, -N., Omar, -A. 2013. Techniques for extraction of bioactive compounds from plant materials: a review. Journal of Food Engineering. 117(4), 426-436. http://fac.ksu.edu.sa/sites/default/files/23-jfe_117_2013.pdf

Bae, -H., Jayaprakasha, -G.K., Jifon, -J., Patil, -B.S., 2012. Variation of antioxidant activity and the levels of bioactive compounds in lipophilic and hydrophilic extracts from hot pepper (Capsicum spp.) cultivars. Food Chemistry. 134(4), 1912-1918. https://doi.org/10.1016/j.foodchem.2012.03.108

Barbero, -G., Liazid, -A., Palma, -M., Barroso, C. 2008. Ultrasound-assisted extraction of capsaicinoids from peppers. Talanta. 75(5), 1332-1337. https://doi.org/10.1016/j.talanta.2008.01.046

Boonkird, -S., Phisalaphong, -C., Phisalaphong, -M., 2008. Ultrasound-assisted extraction of capsaicinoids from Capsicum frutescens on a lab-and pilot-plant scale. Ultrasonics Sonochemistry. 15(6):1075-1079. https:// doi.org 10.1016/j.ultsonch.2008.04.010

Dahmoune, -F., Spigno, -G., Moussi, -K., Remini, -H., Cherbal, -A., Madani, -K., 2014. Pistacia lentiscus leaves as a source of phenolic compounds: Microwave-assisted extraction optimized and compared with ultrasound-assisted and conventional solvent extraction. Industrial Crops and Product. 61, 31-40. https://doi.org/10.1016/j.indcrop.2014.06.035

De Aguiar, -A.C., Sales, -L.P., Coutinho, -J.P., Barbero, -G.F., Godoy, -H.T., Martínez, -J., 2013. Supercritical carbon dioxide extraction of Capsicum peppers: global yield and capsaicinoid content. The Journal of Supercritical Fluids. 81, 210-216. https://doi.org/10.1016/j.supflu.2009.07.008

Depkes. 2005. Daftar Komposisi Bahan Makanan. Piranti Lunak NutriClin versi 2.0 edisi kedua, Subdit Gizi Klinis, Departemen Kesehatan Indonesia, Jakarta

Emmanuel-Ikpeme, -C., Henry, -P., Okiri, -O. A. 2014. Comparative evaluation of the nutritional, phytochemical and microbiological quality of three pepper varieties. Journal of Food Nutrition and Sciences. 2(3), 74-80. https://doi.org/10.11648.j.jfns.20140203.15

Gurnani, -N., Gupta, -M., Shrivastava, -R., Mehta, -D., Mehta, -B.K., 2016. Effect of extraction methods on yield, phytochemical constituents, antibacterial and antifungal activity of Capsicum frutescens L. Indian Journal of Natural Products and Resources (IJNPR)[Formerly Natural Product Radiance (NPR)]. 7(1), 32-39. http://op.niscair.res.in/index.php/IJNPR/article/viewFile/9138/700

Irfan, -I., Pawelzik, -E., Luecke, -W. 1998. The use of microwave to inativate enzymes in rape seeds. Proceedings of the 7th International Working Conference on Stored product Protection. 7(2):1667-1670. https://spiru.cgahr.ksu.edu/proj/iwcspp/pdf2/7/1667.pdf

Kim, -K.S., Koo, -M.S., Jeon, -J.W., Park, -H.S., Seung, -I.S., 2002. Capsicum plaster at the Korean hand acupuncture point reduces postoperative nausea and vomiting after abdominal hysterectomy. Anesthesia & Analgesia. 95(4), 1103-1107. https://doi.org/10.1097/00000539-200210000-00059

Knotkova, -H., Pappagallo, -M., Szallasi, -A. 2008. Capsaicin (TRPV1 Agonist) therapy for pain relief: farewell or revival?. The Clinical Journal of Pain. 24(2), 142-154. https://doi.org/AJP.0b013e318158ed9e

Leong, -T., Ashokkumar, -M., Kentish, -S., 2011. The fundamentals of power ultrasound—a review. Acoust. Aust. 39(2):54-63. http://hdl.handle.net/11343/123494

Luo, -X.J., Peng, -J., Li, -Y.J., 2011. Recent advances in the study on capsaicinoids and capsinoids. European Journal of Pharmacology. 650(1), 1-7. https://doi.org/10.1016/j.ejphar.2010.09.074

Mikamo E, -Y. Okada, -A. Semma, -Y. Otto, Morimoto, -I. 2000. Studies on structural correlation-ship with antioxidant activity of flavonoids. Japanees Journal of Food Chemistry and safety. 7(2), 93-96. https://doi.org/10.18891/jjfcs.7.2_93

Nascimento, -P.L., Nascimento, -T.C., Ramos, -N.S., Silva, -G.R., Gomes, -J.E.G., Falcão, -R.E., Moreira, -K.A., Porto, -A.L., Silva, -T., 2014. Quantification, antioxidant and antimicrobial activity of phenolics isolated from different extracts of Capsicum frutescens (Pimenta Malagueta). Molecules. 19(4), 5434-5447. https://doi.org/10.3390/molecules19045434

Njoku, -P.C., Ayuk, -A.A., Okoye, -C.V., 2011. Temperature effects on vitamin C content in citrus fruits. Pakistan Journal of Nutrition. 10(12), 1168-1169. https://doi.org/10.3923/pjn.2011.1168.1169

Park, -H.S., Kim, -K.S., Min, -H.K., Kim, -D.W., 2004. Prevention of postoperative sore throat using capsicum plaster applied at the Korean hand acupuncture point. Anaesthesi. 59(7):647-651. https://doi.org./10.1111/j.1365-2044.202004.03739.x

Pradana, -F. A., Khoirul, -M. D., Septian -A. 2017. The effectiveness Test of Ultrasonic Extraction Microwave Distillation (USE-MD) Method in Capsaicin Extraction. IPTEK, Journal of Engineering. 3(1), 6-10. https://doi.org/ 10.12962/joe.v3i1.2265

Rahmawati, -A., Putri, -W.D.R. 2013. Karakteristik ekstrak kulit jeruk bali menggunakan metode ekstraksi ultrasonik (kajian perbandingan lama blansing dan ekstraksi). Jurnal Pangan dan Agroindustri. 1(1). 26-35. https://jpa.ub.ac.id/index.php/jpa/article/view/3/3

Selimović, -A., Salkić, -M., Selimović, -A., 2011. Direct spectrophotometric determination of L-ascorbic acid in pharmaceutical preparations using sodium oxalate as a stabilizer. International Journal of Basic & Applied Sciences. 11(02), 106-109. https://doi.org/10.1.1.419.9203

Sricharoen, -P., Lamaiphan, -N., Patthawaro, -P., Limchoowong, -N., Techawongstien, -S., Chanthai, -S., 2016. Phytochemicals in Capsicum oleoresin from different varieties of hot chilli peppers with their antidiabetic and antioxidant activities due to some phenolic compounds. Ultrasonics Sonochemistry. 38,629-639. https://doi.org/10.1016/j.ultsonch.2016.08.018

Vega-Gálvez, -A., Di Scala, -K., Rodríguez, -K., Lemus-Mondaca, -R., Miranda, -M., López, J., Perez-Won, M. 2009. Effect of air-drying temperature on physico-chemical properties, antioxidant capacity, colour and total phenolic content of red pepper (Capsicum annuum, L. var. Hungarian). Food Chemistry.117(4):647-653. http://agris.fao.org/agris-search/search.do?recordID=US201301652895

Victorija -M., Lilijana -K.G., Tatjana -R., Cvetanovska -A., Rubin -G. 2013. Antioxidative effect of Capsicum oleoresins compare with pure capsaicin. Journal of Pharmacy. 4(11), 44-48. https://10.9790/3013-0411044048

Wang, -Y.L., Xi, -G.S., Zheng, -Y.C., Miao, -F.S. 2010. Microwave-assisted extraction of flavonoids from Chinese herb Radix puerariae (Ge Gen). Journal of Medicinal Plants Research. 4(4), 304-308. http://www.academicjournals.org/app/webroot/article/article1380526817_Wang%20et%20al.pdf

Whiting, -S., Derbyshire, -E. Tiwari, -B. 2012. Capsaicinoids and capsinoids. A potential role for weight management? A systematic review of the evidence. Appetite. 59(2), 341-348. https://doi.org/10.1016/j.appet.2012.05.015

Xu, -D.P., Zhou, -Y., Zheng, -J., Li, -S., Li, -A.N., Li, -H.B., 2015. Optimization of ultrasound-assisted extraction of natural antioxidants from the flower of Jatropha integerrima by response surface methodology. Molecules. 21(1), 18. https://doi.org/10.3390/molecules 21010018

Yang, -Z.H., Wang, -X.H., Wang, -H.P., Hu, -L.Q., Zheng, -X.M., Li, -S.W., 2010. Capsaicin mediates cell death in bladder cancer T24 cells through reactive oxygen species production and mitochondrial depolarization. Urology. 75(3), 735-741. https://doi.org/10.1016/j.urology.2009.03.042

Zeleny, -M. 1982. Multiple Criteria Decision Making. Mc Graw Hill, New York

Zhang, -G., He, -L. Hu, -M., 2011. Optimized ultrasonic-assisted extraction of flavonoids from Prunella vulgaris L. and evaluation of antioxidant activities in vitro. Innovative Food Science & Emerging Technologies. 12(1):18-25. https//doi.org/10.1016/j.ifset.2010.12.003

Zhuang, -Y., Chen, -L., Sun, -L. and Cao, -J., 2012. Bioactive characteristics and antioxidant activities of nine peppers. Journal of Functional Foods. 4(1), 331-338. https://doi.org/10.1016/j.jff.2012.01.001

Zimmer, -A.R., Leonardi, -B., Miron, -D., Schapoval, -E., De Oliveira, -J.R., Gosmann, -G. 2012. Antioxidant and anti-inflammatory properties of Capsicum baccatum: from traditional use to scientific approach. Journal of Ethnopharmacology. 139(1), 228-233. https://doi.org/10.1016/

j.jep.2011.11.005

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Published

2019-08-01

Issue

Vol. 20 No. 2 (2019)

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