• Diding Suhandy Jurusan Teknik Pertanian – Fakultas Pertanian –Universitas Lampung Jl. Prof. Dr. Sumantri Brojonegoro No. 1 Bandar Lampung, 35145
  • Meinilwita Yulia Program Studi Mekanisasi Pertanian – Jurusan Teknologi Pertanian –Politeknik Negeri Lampung Jl. Soekarno-Hatta No.10, Rajabasa, Bandar Lampung, Lampung Indonesia, 35141




Akurasi Klasifikasi, LDA, Madu Acacia Mangium, Pemalsuan Madu, Uji Keaslian



 Produksi madu uniflora cukup terbatas, khususnya yang dihasilkan oleh lebah hutan Apis dorsata. Madu uniflora yang dihasilkan lebah hutan Apis dorsata dari nektar bunga pohon Akasia Mangium (Acacia mangium) merupakan salah satu madu hutan premium yang sangat populer di Indonesia dan Malaysia. Studi uji keaslian madu uniflora Acacia mangium menggunakan spektroskopi ultraviolet belum dilakukan. Penelitian ini dilakukan untuk mengevaluasi penggunaan spektroskopi ultraviolet dan kemometrika untuk membedakan madu uniflora Acacia mangium dengan madu multiflora yang memiliki warna mirip madu uniflora Acacia mangium. Sebanyak  50  sampel  madu  uniflora Acacia mangium  dan  50  sampel madu   multiflora disiapkan sebagai sampel.  Sampel tersebut disiapkan dengan cara mencampurkan madu dan air distilasi dengan perbandingan 1:30 (volume/volume). Spektra  seluruh sampel madu dan referensi (air distilasi) di panjang gelombang 190-1100 nm (full spectrum) diakuisisi  menggunakan spektrometer UV-visible (Genesys™  10S  UV-Vis,   Thermo   Scientific,   USA). Klasifikasi tidak terbimbing menggunakan metode HCA dan PCA menunjukkan sampel madu dapat dikelompokkan ke dalam dua kluster berbeda yaitu kluster madu uniflora dan kluster madu multiflora. Klasifikasi terbimbing menggunakan metode LDA menunjukkan hasil yang sangat memuaskan dengan nilai akurasi sebesar 100% berhasil diperoleh baik untuk kalibrasi maupun prediksi.

Kata kunci: Akurasi Klasifikasi; LDA; Madu Acacia Mangium; Pemalsuan Madu; Uji Keaslian



The production of monofloral honey is quite limited, especially that produced by Apis dorsata forest honeybees. The monofloral honey produced by Apis dorsata bees from the flower nectar of the Acacia mangium tree (Acacia mangium) is one of the premium forest honey which is very popular in Indonesia and Malaysia. The study of the authenticity of Acacia mangium monofloral honey using UV spectroscopy has not been carried out. This research was conducted to evaluate the use of UV spectroscopy and chemometrics to discriminate monofloral Acacia mangium honey from multifloral honey which has a similar color to Acacia mangium monofloral honey. A total of 50 samples of Acacia mangium monofloral honey and 50 samples of multifloral honey were prepared as samples. The sample was prepared by mixing honey sample and distilled water in a ratio of 1:30 (volume / volume). Spectra of all honey and reference samples (distilled water) at 190-1100 nm (full spectrum) wavelengths were acquired using a UV-visible spectrometer (Genesys â„¢ 10S UV-Vis, Thermo Scientific, USA). Unsupervised classification using the HCA and PCA methods indicated that the honey samples could be grouped into two different clusters, namely the monofloral honey cluster and the multifloral honey cluster. Supervised classification using the LDA method shows very satisfying results with an accuracy value of 100% successfully obtained for both calibration and prediction.

Keywords : Acacia Mangium Honey; Authentication; Classification Accuracy; Honey Adulteration; LDA


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