KOMPONEN BIOAKTIF, AKTIVITAS ANTIOKSIDAN DAN PROFIL ASAM LEMAK EKSTRAK RIMPANG JERINGAU MERAH (ACORUS SP) DAN JERINGAU PUTIH (ACORUS CALAMUS)

Authors

  • Ariya Sofyan Universitas Brawijaya
  • Eko Widodo
  • Halim Natsir

DOI:

https://doi.org/10.21776/ub.jtp.2017.018.03.17

Keywords:

Antioksidan, Asam lemak, Flavonoid, Jeringau

Abstract

ABSTRAK

Jeringau merah (Acorus sp) dan jeringau putih (Acorus calamus) merupakan tanaman dari famili Araceae yang rimpangnya dijadikan bahan obat-obatan. Antioksidan merupakan senyawa yang dapat menghambat reaksi oksidasi, dengan mengikat radikal bebas dan molekul yang sangat reaktif sehingga kerusakan sel akan dihambat. Penelitian ini bertujuan untuk menganalisis senyawa bioaktif, aktivitas antioksidan dan profil asam lemak pada jeringau merah dan jeringau putih sehingga dapat digunakan sebagai bahan tambahan dalam makanan, pakan serta sebagai sumber antioksidan alami. Bahan yang digunakan dalam penelitian ini adalah rimpang jeringau merah dan jeringau putih. Hasil analisis menunjukan kandungan flavonoid dan aktivitas antioksidan (IC50) pada jeringau merah masing-masing sebesar 33.76 % b/b dan 0.22 mg/ml sedangkan tepung rimpang jeringau putih sebesar 3.84 % b/b dan 0.49 mg/ml. Jenis asam lemak yang dominan menyusun fraksi lipida rimpang jeringau merah dan jeringau putih adalah laurat dan palmitat (unsaturated fatty acid). Asam lemak jenuhnya terdiri dari 7 macam asam lemak dengan asam palmitat sebagai komponen utamanya. Sementara itu, pada rimpang jeringau merah mengandung asam linoleat, sedangkan pada jeringau putih tidak ada. Penelitian ini menunjukkan bahwa rimpang jeringau merah dan jeringau putih dapat digunakan sebagai bahan tambahan dalam makanan, pakan, serta sebagai sumber antioksidan alami

 

ABSTRACT

Red cypress (Acorus sp) and white jeringau (Acorus calamus) are plants of the Araceae family whose rhizome is made into medicinal materials. Antioxidants are compounds that can inhibit oxidation reactions, by binding to free radicals and highly reactive molecules so that cell damage will be inhibited. This study aims to analyze bioactive compounds, antioxidant activity and fatty acid profiles in red and white jinks and white jeringau so it can be used as an additive in food, feed as well as a source of natural antioxidants. The materials used in this study are red jisan rhizome and white jeringau. The results showed that flavonoid content and antioxidant activity (IC50) in red jeringau were 33.76% w / w and 0.22 mg /ml respectively, while white jengese rhizome flour was 3.84% w / w and 0.49 mg / ml. The dominant types of fatty acids that make up the fraction of red jelly rhizome and white jeringau are lauric and palmitate (unsaturated fatty acid). The saturated fatty acid consists of seven kinds of fatty acids with palmitic acid as its main component. Meanwhile, the red jisanau rhizome contains linoleic acid while in white jinkau not exist. This study shows that red jisan rhizome and white jinkau can be used as additive in food, feed as well as natural source of antioxidant

References

AOAC. 2005. Official Method of Analysis of the Association of Official Analytical. Chemist. Benyamin Franklin Station. Washington, D.C

Andersson, C, M, Hallberg, A, Högberg, T. 1996. Advances in the development of pharmaceutical antioxidants. Advances in Drug Research. 28:65-180

Atanassova, M, Georgieva, S, Ivancheva, K. 2011. Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medical herbs. Journal of the University of Chemical Technology and Metallurgy. 46(1):81-88

da Rosa, G, A, Sorbello, L, A, Dittrich, R, L, de Moraes, M, T, T, de Oliveira, E, G. 2011. Blood profile of Japanese quail (Coturnix japonica) under thermal stress. Ciência Rural. 41(9):1605-1610

Buttriss, J. 1989. Free radicals. Nutrition & Food Science. 89(1):12-13

BPPT. 2017. Jeringau (Acorus Calamus L) sebagai fungisida. Dilihat 27 Juli 2017. <http://www.kelair.bppt.go.id/sib-3pop/Iptek/Jeringau/Jeringau.htm>

Cao, H, Xie, Y, Chen, X. 2015. Type 2 diabetes diminishes the benefits of dietary antioxidants: Evidence from the different free radical scavenging potential. Food Chemistry. 186:106-112

Duthie, G, G. 1990. Antioxidant Vitamins, free radicals and coronary heart disease. British Food Journal. 92(8):32-36

Erguder, B, I. Avci, A, Devrim, E, Durak, I. 2007. Effects of cooking techniques on antioxidant enzyme activities of some fruits and vegetables. Turk. J. Med. Sci. 37(3):151-156

Fru-Nji, F, Niess, E, Pfeffer, E. 2007. Effect of graded replacement of soybean meal by faba beans (Vicia faba L.) or field peas (Pisum sativum L.) in rations for laying hens on egg production and quality. The Journal of Poultry Science. 44(1):34-41

Ghiselli, A, Nardini, M, Baldi, A, Scaccini, C. 1998. Antioxidant activity of different phenolics fractions separated from an italian red wine. J. Agric. Food. Chem. 46(2):361-367

Gill, M, I, Tomás-Barberán, F, A, Hess-Pierce, B, Kader, A, A. 2002. Antioxidant capacities, phenolic compounds, carotenoids, and vitamin C contents of nectarine, peach, and plum cultivars from california. J. Agric. Food Chem. 50:4976-4982

Halliwell, B, Gutteridge, JMC. 2000. Free Radical in Biology and Medicine. Oxford University Press, New York

Harahap, G, F, R. 2014. Uji Efektivitas Ekstrak Etanol Daun Ciplukan (Physalis angulata L.) sebagai Antimikroba Terhadap Acinetobacter baumannii secara in Vitro. Skripsi. Universitas Brawijaya. Malang

Hasan, M, U, Sagheer, M, Ullah, E, Ahmad, F, Wakil, W. 2006. Insecticidal activity of different doses of Acorus calamus oil against Trogoderma granarium (everts). Pak. J. Agri. Sci. 43(1-2):55-58

Hasan, M, N. 2015. Pengaruh Ekstrak Rimpang Jeringau (Acorus calamus L.) dalam Beberapa Pelarut Organik Terhadap Aktivitas Antioksidan dan Antifungi secara in Vitro. Tesis. King Mongkut’s University of Technology Thonburi. Thailand

He, X, Xia, Q, Woodling, K, Lin, G, Fu, P, F. 2017. Pyrrolizidine alkaloid-derived DNA adducts are common toxicological biomarkers of pyrrolizidine alkaloid N-oxides. Journal of Food and Drug Analysis. 25(4):984-991

Heim, K, E, Tagliaferro, A, R, Bobilya, D, J. 2002. Flavonoid antioxidant: chemistry, metabolism and structure-activity relationships. The Journal of Nutritional Biochemistry. 13(10):572-584

Holley, K, M, Phillips, P, S. 1995. Trans-fatty acids: an introduction. Nutrition & Food Science. 95(2):31-33

Imam, H, Zamigar, Sofi, G. 2014. Mosquito larvicidal efficay of Acorus calamus extracts against Aedes aegypti L. larvae. Asian Pacific Journal of Tropical Disease. 4:S181-S185

Levetin, E, McMahon, K. 2006. Plant and Society. McGraw-Hill, New York

Liu, H, Liu, W. 2017. Concentration and distributions of fatty acids in algae, submerged plants and terrestrial plants from the northeastern Tibetan Plateau. Organic Geochemistry. 113:17-26

Marinova, D, Ribarova, F, Atanassova, M. 2005. Total phenolics and total flavonoids in bulgarian fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy. 40(3):255-260

Mahmoudi, S, Khali, M, Benkhaled, A, Benamirouche, K, Baiti, I. 2016. Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaf extracts from ten Algerian Ficus carica L. varieties. Asian Pacific Journal of Tropical Biomedicine. 6(3):239-245

Maududi, A, A. 2009. Produksi Antioksidan dari Daun Simpur (Dillenia indica) menggunakan Metode Ekstraksi Tekanan Tinggi dengan Sirkulasi Pelarut. Skripsi. Universitas Indonesia. Jakarta

Molyneux, P. 2003. The Use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology. 26(2): 211-219

Murphy, D, J. 2017. Alkaloids, Encyclopedia of Applied Plant Sciences (Second Edition). Elsevier, USA

Nagota, Y, Sakamoto, K, Shiratsuchi, H, Ishii, T, Yano, M, Ohta, H. 2006. Flavonoid composition of fruit tissue of citrus species. Biosci. Biotechnol. Biochem. 70:178-192

Ningsih, I, Y, Zulaikhah, S, Hidayat, M, A, Kuswandi, B. 2016. Antioxidant activity of various kenitu (Chrysophyllum Cainito L.) leaves extracts from jember, indonesia. Agriculture and Agricultural Science Procedia. 9:378-385

Ossia, C, V, Han, H, G, Kong, H. 2010. Tribological evaluation of selected biodegradable oils with long chain fatty acids. Industrial Lubrication and Tribology. 62(1):26-31

Oversen, L, Leth, T. 1995. Trans fatty acids: time for legislative action?. Nutrition & Food Science. 95(3):16-19

Palupi, R, Abdullah, L, Astuti, D, A, Sumiati. 2014. Potential and utilization of Indigofera sp. shoot leaf meal as soybean meal substitution in laying hen diets. Jurnal Ilmu Teknik dan Veteriner. 19(3):210-219

Paradis, A, J, Nawar, W, W. 1981. A gas chromatographic method for assement of used frying oils comparation of other methods. Journal of the American Oil Chemists’ Society. 58(5):635-638

Qi, K, K, Chen, J, L, Zhao, G, P, Zheng, M, Q, Wen, J. 2010. Effect of dietary x6/x3 on growth performance, carcass traits, meat quality and fatty acid profiles of Beijing-you chicken. Journal of Animal Physiology and Animal Nutrition. 94(4):474-485

Rajput, S, B, Tonge, M, B, Karuppayil, S, M. 2014. An overview on traditional uses and pharmacological profile of Acorus calamus Linn. (Sweet flag) and other Acorus species. Phytomedicine. 21(3):268-276

Sardarodiyan, M, Sani, A, M. 2016. Natural antioxidants: sources, extraction and application in food systems. Nutrition & Food Science. 46(3):363-373

Sharma, O, P, Bhat, T, J. 2009. DPPH antioxidant assay revisited. Food Chemistry. 113(4):1202-1205

Silva, F, A, Borges, F, Guimaraes, C., Lima, J, L, Matos, C, Reis, S. 2000. Phenolic acids and derivatives: studies on the relationship among structure, radical scavenging activity, and physicochemical parameters. J. Agric. Food Chem. 48(6):2122-2126

Simanjorang, J. 2008. Efektivitas Rimpang Jeringau (Acorus calamus L.) dalam Membunuh Nyamuk Aedes aegypti. Skripsi. Universitas Sumatera Utara. Medan

Supriyantini, E, Widowati, I, Ambariyanto. 2007. Kandungan asam lemak omega-3 (asam linolenat) pada kerang totok Polymesoda erosa yang diberi pakan Tetraselmis chuii dan Skeletonema costatum. Ilmu Kelautan. 12 (2):97-104

Utari, CR, S, Widyarini, T, Sutarmiadji, D, Dharmawan, R, Darukutni. 2006. Potensi minyak rimpang jeringau (Acorus calamus L.) sebagai repelen terhadap nyamuk Culex quinquefasciatus L. Biofarmasi. 4(1):1-3

Viswanad, V, Aleykutty, N, A, Zacharia, S, M, Thomas, L. 2011. Evaluation of antioxidant and free radical scavenging activity of Samadera indica using in vitro models. Pharmacognosy Journal. 3(23):85-90

Wojtunik-Kulesza, K, A, Oniszczuk, A, Oniszczuk, T, Waksmundzka-Hajnos, M. 2016. The influence of common free radicals and antioxidants on development of Alzheimer’s disease. Biomedicine & Pharmacotherapy. 78:39-49

Wu, J, Q, Kosten, T, R, Zhang, X, Y. 2013. Free radicals, antioxidant defense systems, and schizophrenia. Progress in NeuroPsychopharmacology and Biological Psychiatry. 46:200-206

Zabri, H, Kodjo, C, Benie, A, Bekro, J, M, Bekro, Y, A. 2008. Phytochemical screening dandetermination of flavonoids in Secamone afzelii (Asclepiadaceae) extracts. African Journal of Pure and Applied Chemistry. 2(8):080-082

Zhang, S, H, Zhang, S, Y, Li, G. 2016. Acorus calamus root extracts to control harmful cyanobacteria blooms. Ecological Engineering. 94:95-101

Zheng, W, Wang, S, Y, 2001. Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chem. 49(11):5165-

Downloads

Published

2017-12-05

Issue

Section

Articles